RGS5在乳腺癌组织和细胞系中的表达和定位
孙舟彤;何妍彦;张红艳
【期刊名称】《现代肿瘤医学》 【年(卷),期】2024(026)023
【摘要】目的:探讨G蛋白信号转导调节蛋白5(RGS5)在人乳腺癌细胞系中的表达.方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,ZR-75-30,MDA-MB-231,HCC1937的总蛋白和总RNA,提取细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测RGS5的表达.共聚焦激光扫描显微镜观察RGS5胞内定位;免疫组化检测RGS5在乳腺肿瘤组织中表达情况.结果:MDA-MB-231细胞系中RGS5基因表达上调,MCF7和ZR-75-1细胞系中RGS5蛋白表达提高.结论:RGS5在乳腺癌细胞的高表达可能参与其生长和转移,揭示RGS5可能是人乳腺肿瘤的治疗靶点.%Objective:To investigate the expression of RGS5 protein and gene in human breast cancer cell lines. Methods:Western blot analysis was used to determine RGS5 protein expression in breast cancer cell lines,Real-time PCR analysis for exogenous RGS5 expression from breast cancer cell lines. Results:RGS5 gene was higer ex-pressed in MDA-MB-231 than the other cell lines and RGS5 protein was higher expressed in MCF7 and ZR-75-1. Conclusion:RGS5 protein was expressed in breast cancer cell lines,which may be one of the mechanisms that at-tribute to generation and metastasis of cancer cells. 【总页数】4页(3713-3716)
RGS5在乳腺癌组织和细胞系中的表达和定位
