Propofol may protect PC12 ceils from β-amyloid25-35 induced apoptosis through the GSK-3β

Propofol may protect PC12 ceils from β-amyloid25-35 induced apoptosis through the GSK-3β signaling pathway

ZHANG Rui;XU Jie;LIU Yan-yong;ZUO Ping-ping;YANG Nan;JI Chao;WANG Yun

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2013(126)010

【摘要】Background There are two major pathological hallmarks of AIzheimer's disease.One is the progressive accumulation of beta-amyloid (Aβ) in the form of senile plaques； the other is hyperphosphorylated tau,causing neuronal apoptosis.Some inhalation anesthetics,such as isoflurane and desflurane,have been suggested to induce Aβ accumulation and cause AD-like neuropathogenesis.Whether intravenous anesthetics have similar effects is still unclear.We therefore set out to determine the relationship between propofol and AD-like pathogenesis.Methods PC12 cells were cultured in serum-free medium for 12 hours prior to drug treatment.Various concentrations from 5 μmol/L to 80 μmol/L of aggregated Aβ25-35 were added to determine a proper concentration for further study.After exposure to 10 μmol/L Aβ25-35 alone or with 20 μmol/L propofol for 6 hours,PC12 cell viability was

determined

by

MTT

assay.Western

blotting

and

immunocytochemical staining were performed to observe the protein expression of the Bcl-2 family,tau phosphorylation at different sites,and