Phylogenetic relationships of host insects of Cordyceps sinensis inferred from mitochondri

Phylogenetic relationships of host insects of Cordyceps sinensis inferred from mitochondrial

Cytochrome b sequences

Cheng Zhou;Geng Yang;Liang Honghui;Yang Xiaoling;Li Shan;Zhu Yunguo;Guo Guangpu;Zhou Tongshui;Chen Jiakuan

【期刊名称】《自然科学进展（英文版）》 【年(卷),期】2007(017)007

【摘要】This study used the sequence of the mitochondrial Cytochrome b (Cytb) to estimate phylogenetic relationships among host Hepialidae insects of Cordyceps sinensis. Genome DNA of host insect was extracted from the dead larva head part of 18 cordyceps populations and 2 species of Hepialus, and the Cytb fragment of host insect was amplified with PCR technique. The nucleotide sequence alignments and their homologous sequences of 24 species host Hepialidae insects of Cordyceps sinensis were obtained from GenBank and were used to construct phylogenetic trees based on neighbor-joining method. The results showed that genus Bipectilus diverged earlier than genus Hepialus and Hepialiscus. Hepialus host insects of Cordyceps sinensis have multitudinous species with different morphological characteristics and geographical distributions. The interspecific genetic differentiations are obvious in Hepialus. Thus, the genus Hepialus might be considered as polyphyletic origin. Cytb sequences have abundant variations among