HPLC法测定前列康复胶囊中大车前苷、朝藿定C和淫羊
藿苷
李征;罗晶;潘延宁
【期刊名称】《现代药物与临床》 【年(卷),期】2015(000)008
【摘要】Objective To establish a method for simultaneous determination of plantamajoside, epimedin C, and icariin in Qianlie Kangfu Capsules. Methods The determination was carried out on Agilent Zorbax C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 ml/min. The column temperature was set at 35 ℃ and the detection wavelengths were 330 nm in 0—30 min (determination of plantamajoside) and 270 nm in 30—50 min (determination of epimedin C and icariin). Results Plantamajoside, epimedin C, and icariin had good linearity in the ranges of 10.08 — 201.60 ng, 18.43 — 368.60 ng, and 52.25 —1 045.00 ng, respectively. The average recoveries were 100.6%, 97.2%, and 97.2%with RSD 1.5%, 1.3%, and 1.2%, respectively. Conclusion The method is simple and repeatable, which can be applied to the quality control for Qianlie Kangfu Capsules.%目的：建立同时测定前列康复胶囊中大车前苷、朝藿定C、淫羊藿苷的HPLC法。方法安捷伦Zorbax C18色谱柱（250 mm×4.6 mm，5μm）；以乙腈–0.1%磷酸水溶液为流动相，梯度洗脱；柱温35℃；检测波长
HPLC法测定前列康复胶囊中大车前苷、朝藿定C和淫羊藿苷
