Cloning,expression pattern,and potential role of MAPKp38 and NF-κBp65 in response to lipo

Cloning,expression pattern,and potential role of

MAPKp38 and NF-κBp65 in response to lipopolysaccharide in yellow catfish(Pelteobagrus

fulvidraco)

Dan Shi; Ye Zhao; Lin Feng; Yang Liu; Wei-Dan Jiang; Pei Wu; Juan Zhao; Jun Jiang; Xiao-Qiu Zhou

【期刊名称】《动物营养：英文版》 【年(卷),期】2019(000)004

【摘要】Mitogen-activated protein kinases(MAPK) and nuclear factor kappa B(NF-κB) pathways are considered to be two crucial intracellular signaling cascades in pro-inflammatory responses.In this study,we reported the coding sequences(CDS) of MAPKp38 and NF-κBp65 from yellow catfish.We also investigated the gene structure,expression patterns and functional role in yellow catfish.The CDS of MAPKp38 is1,086 bp encoding 361 amino acids(AA).The MAPKp38 protein has a long highly conserved serine/threonine protein kinases catalytic domain.The NF-κBp65 CDS is 1,794 bp,and the gene encodes 597 AA,with a Rel homology domain(RHD) which consists of a RHD-DNA-binding domain and an Ig-like,plexins,transcription factors(IPT) domain.Moreover,MAPKp38 and NF-κBp65 protein of bony fish and other vertebrates have a single clade.Quantitative real-time PCR analysis revealed the presence of the MAPKp38 and NF-κBp65 transcript in 12