异丙酚对大鼠内毒素脑损伤磷酸化JAK2、STAT3表达的
影响
邓必高;但伶;黄燕
【期刊名称】《中国医院药学杂志》 【年(卷),期】2011(031)022
【摘要】目的:探讨异丙酚对大鼠内毒素脑损伤中磷酸化JAK2、STAT3蛋白表达的影响.方法:健康清洁级SD大鼠72只,雌雄不限,体质量220～250 g,随机分为3组(n=24):L组(内毒素组)和LP组(内毒素+异丙酚组)经颈内动脉注射内毒素200μg建立大鼠感染性脑损伤模型,C组(对照组)经颈内动脉注射等量生理盐水,LP组颈内动脉注射内毒素后即予异丙酚100mg·kg-1剂量腹腔注射.3组分别于6,12,24,48 h随机处死6只大鼠,取额叶皮质,检测脑组织含水量、磷酸化JAK2和STAT3蛋白表达水平,光镜下观察脑组织形态及病理变化.结果:与C组相比,L组、LP组各时间点脑组织含水量、P JAK2和P-STAT3表达增加(P＜0.05,P＜0.01)；与L组比较,LP组各时间点脑含水量、P-JAK2和P-STAT3表达明显减少(P＜0.05,P＜0.01).结论:异丙酚减轻大鼠内毒素性脑损伤的机制可能与抑制脑组织磷酸化JAK2、STAT3表达水平上调有关.%OBJECTIVE To investigate the effects of propofol on lipopolysaccharide (LPS) induced activation of P-JAK2 and P-STAT3 in brain tissue in rats. METHODS Seventy two SD rats of both sexes weighing 220 - 250 g were randomly divided into 3 groups(n = 24 each): Cerebral edema was induced by injecting LPS 200 μg via left internal carotid artery in group L and group LP. In control group (group C), equal volume of nomad saline was
administered iv instead of LPS. Propofol (100 mg·kg-1 ) was given intraperitoneally(IP) immediately after the LPS was given in group LP. Six rats of each group were decapitated at 6,12.24,48 h after administration. The frontal lobe cortexes were immediately removed for the determination of water content, expression of P-JAK2 and P-STAT3 protein, meanwhile pathological change was observed with light microscope. RESULTS In group L and group LP the brain water content,the expression of P-JAK2 and P-STAT3 proteins were significantly increased compared with group C(F<(). 05,P<0. 01). In group LP these results were markedly reduce compared with group L (P<0. 05,P<0. 01). CONCLUSION Propofol can attenuate LPS-induced brain injury, which might be related to the decrease of P-JAK2 and P-STAT3 proteins expression. 【总页数】4页(1867-1870)
【关键词】异丙酚;感染性脑损伤;脂多糖;蛋白质酪氨酸激酶 【作者】邓必高;但伶;黄燕
【作者单位】重庆医科大学附属第二医院麻醉科,重庆400010;重庆医科大学附属第二医院麻醉科,重庆400010;重庆医科大学附属第二医院麻醉科,重庆400010
【正文语种】中文 【中图分类】R641 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-hospital-pharmacy_thesis/0201242985343.html 【相关文献】
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异丙酚对大鼠内毒素脑损伤磷酸化JAK2、STAT3表达的影响
