尼古丁在人椎间盘髓核细胞退变中的作用
黄宇峰;雍之瑶;刘晓明;李浩曦;王善金;巴兆玉;沈彬;赵卫东;吴德升
【期刊名称】《生物骨科材料与临床研究》 【年(卷),期】2017(014)001
【摘要】Objective To evaluate the effect of nicotine on the degeneration of human nucleus pulposus cells.Methods 5 patients undergoing lumbar discectomy surgeries in our department were randomly included.The nucleus pulposus tissues were cultured in well plate and the concentrations of nicotine were 0,1,33.3,100,200,500ng/mL.After 1,2,3,7,10 days,RNA of cells were extracted using Trizol.The mRNA expression ofAQP1,AQP3,collagen Ⅱ and Aggrecan were valued by rtPCR.Data analysis was done by SPSS19.0.Results With an increase in nicotine concentration and culture time,the morphology of nucleus pulposus
changed
significantly,weakening
of
the
adherent
ability,showing decrease of cytoplasm,atrophy of cell nucleus and increase ofapotosis.The mRNA expression ofAQP 1 and AQP3 also decreased and so did collagen Ⅱ and aggrecan.Conclusion Nicotine can decrease the mRNA expression of AQP 1and AQP3 of nucleus pulposus,thus,causing a decrease in the expression of type Ⅱ collagen and aggrecanto accelerate disc degeneration.This effect seems to be time and dose dependent.%目的 探讨不同浓度及时间的尼古丁在人椎间盘髓核细胞退变中的作用.方法 随机选择5例腰椎骨折患者的椎间盘髓核组织进
尼古丁在人椎间盘髓核细胞退变中的作用
