纤维素酶辅助提取油菜籽皮中的原花青素

纤维素酶辅助提取油菜籽皮中的原花青素

秦永剑;张加研;万永燕

【期刊名称】《北京农业》 【年(卷),期】2012(000)024

【摘要】 以油菜籽皮为原料,采用纤维素酶辅助提取原花青素.以原花青素提取率为指标,考察油菜籽皮粒径、纤维素酶用量、酶解时间对提取率的影响,采用正交试验对工艺条件进行优化,试验得出最佳工艺条件为：油菜籽皮粒径90目,纤维素酶用量35 U/g(FPA),酶解时间100 min,该工艺条件下的原花青素提取率为2.90%,与未添加纤维素酶相比,原花青素提取率提高了38.76%.% The extraction of procyanidins from rapeseed hull by using cellulose-assistant extraction technology. The effects of particle size, extraction time and cellulose amount with extraction rate of procyanidins as selection standard. Orthogonal design adopted to optimize extraction conditions. The results of the optimum extraction conditions were as follows:the extracted particle size used was between 160~180 μm,the added amount of cellulase was 35 u(FPA)/g(rapeseed hull),and the cellulase extraction time was 100 min. Using the mentioned optimum conditions, the extraction rate of procyanidins was 2.90%and increased by 38.76%, compared with the non-using the cellulase-assistant technology.

【总页数】3页(4-5,6)

【关键词】油菜籽皮;原花青素;纤维素酶;提取