Inhibitory activities of plumbagin on cell migration and invasion and inducing activity on

Inhibitory activities of plumbagin on cell migration

and invasion and inducing activity on cholangiocarcinoma cell apoptosis

Luxsana

Panrit;Tullayakorn

Plengsuriyakarn;Pongsakorn

Na-

Martviset;Kesara

Bangchang

【期刊名称】《亚太热带医药杂志（英文版）》 【年(卷),期】2018(011)007

【摘要】Objective: To investigate the cytotoxic, apoptotic and inhibitory activities on cell migration and invasion of plumbagin in the human cholangiocarcinoma (CCA) cell line (CL-6) in comparison with human embryonic fibroblast cell line (OUMS). Methods: Cytotoxicity activity was evaluated using MTT assay. Inhibitory effect on cell migration and invasion were investigated using label-free real-time cell analysis and QCM ECMatrix cell invasion chamber, respectively. Apoptotic activity was evaluated using flow cytometry and CellEvent? Caspase 3/7 assay. Results: Based on results of the cytotoxicity test in CL-6 cells, 50％ inhibitory concentration (IC50, Mean±SD) values of plumbagin and the standard drug 5-fluorouracil were (24.00±3.33) and (1036.00±137.77) μmol/L, respectively. The corresponding values for OUMS cells were (57.00±5.23) and (2147.00±209.98) μmol/L, respectively. The selectivity index was 2.28. The inhibitory activities of plumbagin on cell migration and invasion were potent and concentration-dependent with IC50 of