Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electroc

Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary

Electrochromatography

ZHENG,Chao;LIU,Zhao-Sheng;GAO,Ru-Yu;ZHANG,Yu-Kui

【期刊名称】《中国化学（英文版）》 【年(卷),期】2008(026)010

【摘要】A tetrapeptide-imprinted capillary monolithic column was synthesized successfully in the presence of YPLG (Tyr-Pro-Leu-Gly) as template by in situ thermo-initiated polymerization. For exploring the recognition mechanism of molecularly imprinted polymer in a capillary electrochromatography (CEC) mode, YPLG and its structural analogue, YPGL (Tyr-Pro-Gly-Leu), differing in amino acid sequence, were used as model compounds. It is hard work to separate the two tetrapeptides by a simple CEC or a capillary electrophoresis mode due to their similar chemical structures and values of pi. However, the results showed that YPLG could be recognized specially by the imprinted monolith. The separation factor (a) and the resolution (Rs) between YPLG and YPGL were 1.73 and 3.72, respectively. The effects of organic modifier concentration, pH value of buffer, salt concentration of buffer and column temperature on the recognition of YPLG and YPGL were investigated. A non-linear Van't Hoff behavior of the template was observed on a YPLG-imprinted column, which suggested the presence

of multiple types of retention mechanisms or multiple types of binding sites. The results showed that the molecular recognition on MIP monolith in the CEC mode mainly derived from hydrogen bonding between YPLG and MIP and three-dimensional structure of the imprinting cavities.

【总页数】6页(1857-1862) 【关键词】molecularly

imprinted

polymer;monolith;capillary

electrochromatography;YPLG (Tyr-Pro-Leu-Gly);recognition mechanism 【作者】ZHENG,Chao;LIU,Zhao-Sheng;GAO,Ru-Yu;ZHANG,Yu-Kui

【作者单位】College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;State Chemistry,Institute

Key of

Laboratory Element

of

Element-organic Chemistry,Nankai

Organic

University,Tianjin 300071,China;State Key Laboratory of Element-organic Chemistry,Institute

of

Element

Organic

Chemistry,Nankai

University,Tianjin 300071,China;National Chromatographic Research and Analysis Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Da 【正文语种】中文 【中图分类】O6 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-

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