雌二醇调节树突状细胞功能参与狼疮鼠的发病机制
姜波;孙凌云;王红
【期刊名称】《中华风湿病学杂志》 【年(卷),期】2008(012)008
【摘要】目的 比较雌二醇(E2)对SLE模型鼠-(NZB×NZW)F1(NZB/w F1)雌鼠发病前后脾脏树突状细胞(DC)的影响,探索雌激素对DC的调节是否和系统性红斑狼疮(SLE)的进展相关.方法 抗CD11c单抗标记的免疫磁珠纯化DC,流式细胞仪检测DC表面分子和细胞因子,混合淋巴细胞反应检测DC对脾脏T细胞的刺激功能,半定量反转录一聚合酶链反应(RT-PCR)法检测DC内雌激素受体(ER)的表达.结果 E2影响狼疮鼠脾脏DC的CD40、白细胞介素(IL)-6、IL-10、IL-12、肿瘤坏死因子(TNF)-α和ERα的表达及刺激活性;枸橼酸他莫西芬(TAM)拈抗E2的作用;E2对不同周龄鼠DC的作用不同.结论 E2通过与DC的ERα结合而调控DC来参与SLE的病理机制,E2对DC的作用和病程进展有关.%Objective To investigate the effect of estrogen on dendritic cells (DCs) and its role in disease progression by comparing the effects of 17β-estradiol (E2) on spleen DCs in systemic lupus erythe matosus (SLE) murine modeI-(NZBxNZW) F1 (NZB/w F1 ) female mice before and after the disease onset.Methods Anti-CD11c antibody labeled magnetic bead was used to purify spleen DCs.Flow cytometry was utilized to detect the co-stimulatory molecules and intracellular cytokines of DCs.Mixed lymphocytes reaction (MLR) was used to measure the stimulatory activity of DCs to T iymphocytes.And semi-quantitative RT-PCR was
雌二醇调节树突状细胞功能参与狼疮鼠的发病机制
