Matrigel对人骨肉瘤动物模型移植瘤形成的影响
张龙;孙晓娟;陈成龙;吕智
【期刊名称】《肿瘤研究与临床》 【年(卷),期】2017(029)011
【摘要】目的 研究Matrigel、常规培养液和磷酸盐缓冲液(PBS)三种细胞混悬液对骨肉瘤动物模型移植瘤形成的影响,分析Matrigel对人骨肉瘤细胞分化与增殖的作用.方法 将24只BALB/c-nu/nu裸鼠随机分为3组,每组8只,将人骨肉瘤细胞株SaOS-2细胞分别混悬于Matrigel胶和RPMI 1640完全培养液1:1混悬液(M组)、RPMI 1640完全培养液(R组)、PBS(P组),以1×106/ml细胞等量注射于裸鼠背部皮下,400μl/只.处理后定期测量各组裸鼠成瘤情况,计算肿瘤体积.5周后,解剖成瘤部位,取出肿瘤组织,4%多聚甲醛溶液固定,石蜡包埋、切片,苏木精-伊红(HE)染色,镜下观察组织学形态.结果 M组8只裸鼠均在0d形成肿块,随时间推移逐渐增大;R组和P组在移植后2~4d部分裸鼠皮下肿块消失,1周后部分裸鼠可再次观察到肿块,并随时间逐步生长.接种5周后,M组肿瘤体积明显大于R组和P组,肿瘤体积分别为(3185±488)、(598±189)、(512±109)mm3,差异有统计学意义(F=85.7,P<0.001).5周后解剖瘤体,M组肿瘤质量大于R组和P组,肿瘤质量分别为(2.22±0.18)、(1.48±0.13)、(1.47±0.17)g,差异有统计学意义(F=37.07,P<0.001).R组和P组在肿瘤体积和质量方面差异均无统计学意义(P>0.05).病理组织学检查结果显示,M组细胞较好地保持骨肉瘤细胞原有的分化程度,R组和P组细胞向低分化转变.结论 Matrigel促进人骨肉瘤细胞在实验动物体内较快增殖,成瘤率高,均一性好,瘤体细胞形态学接近其来源组织,更符合人骨肉瘤发生、发展过程.%Objective To
investigate the effects of cell suspension including Matrigel, normal culture medium and phosphate buffer saline (PBS) on the xenograft model establishment of human osteosarcoma. The function of Matrigel on regulating human osteosarcoma cell differentiation and proliferation was analyzed. Methods Twenty-four BALB/c-nu/nu nude mice were randomly divided into three groups with 8 animals in every group: Matrigel and RPMI 1640 suspension (group M), RPMI 1640 culture medium (group R), PBS (group P). Human osteosarcoma cell-SaOS-2 was suspended in the three groups respectively. 1×106/ml equivalent cell counts were injected into the back of each anesthetized nude mouse (400 μl per mouse). Xenograft tumors were measured at regular intervals and the tumor volume was calculated. After 5 weeks of inoculation, the tumor parts were dissected. Paraffin-embedded sections from xenograft tumor tissues were fixed in 4 % paraformaldehyde and pathological study was made after paraffin embedding and cutting under microscope by HE stains. Results Eight nude mice formed tumor lumps at 0 day in group M and were gradually increased over time. Xenograft tumors of group R and group P disappeared in 2-4 days and some appeared again after 1 week with an increase of tumor size. After 5 weeks, the tumor volume in the group M was significantly larger than that in the group P and group R [(3 185 ± 488), (598 ± 189), (512 ± 109) mm3 respectively,F=85.7,P<0.001].After 5
weeks,tumor body was dissected.The tumor weight in the group M was significantly larger than that in the group P and group R[tumor weight:(2.22 ± 0.18),(1.48 ± 0.13),(1.47 ± 0.17) g respectively, F= 37.07, P< 0.001]. There was no difference between group R and group P in tumor volume and weight(P>0.05).Histopathological analysis showed that cells in the group M could keep original degrees of pathological differentiation in osteosarcoma cells. Besides, cells suspension of culture medium or PBS in the group P and group R were poorly differentiated. Conclusions Matrigel can promote high tumor growth rate and good uniformity of human osteosarcoma cells in experimental animals. The histological state is similar to original structure,which conforms to the occurrence and development of human osteosarcoma. 【总页数】5页(721-725)
【关键词】骨肉瘤;模型,动物;移植;基质胶 【作者】张龙;孙晓娟;陈成龙;吕智
【作者单位】030001 太原,山西医科大学第二临床医学院;02912 美国普罗维登斯布朗大学医学院 美国罗德岛医院骨科;030001 太原,山西医科大学第二临床医学院;030001 太原,山西医科大学第二医院骨科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_cancer-
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