Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E.coli

Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E.coli

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【期刊名称】《自然科学进展（英文版）》 【年(卷),期】2002(012)012

【摘要】Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZ1-2 are isolated from Nicotiana tabacum by RT-PCR and rapid amplification cDNA ends (RACE) method. Analysis of the deduced amino acid sequences encoded by NtFtsZ1-1 and NtFtsZ1-2 indicate that these two proteins possess the typical conservative motifs and GTP binding sites existing in all FtsZ proteins. The existence of putative plastid transit peptide in their N-terminal suggests that there are at least two transit-peptide containing FtsZ proteins in higher plants. Phylogenetic analysis based on amino acid sequences of FtsZ proteins also supports this interference. These two NtFtsZ genes demonstrate a similar expression pattern during the plant development, detected by Northern blot. Expression of NtFtsZ1-1 and NtFtsZ1-2 in E.coli interrupts the normal division process of host cells. These results suggest the diverse functions of FtsZ proteins in higher plants. 【总页数】6页(909-914)

【关键词】NtFtsZ genes;plastid division;Nicotiana tabacum;plastid transit peptide;expression in E.coli