Diagnostic Performance of the GenoType MTBDRplus and MTBDRsl Assays to Identify Tuberculos

Diagnostic Performance of the GenoType MTBDRplus and MTBDRsl Assays to Identify Tuberculosis Drug Resistance in Eastern China

Qiao Liu;Guo-Li Li;Cheng Chen;Jian-Ming Wang;Leonardo Martinez;Wei Lu;Li-Mei Zhu

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2017(130)013

【摘要】Background:The WHO recently has recommended the GenoType MTBDRplus version 1.0 and MTBDRsl version 1.0 assays for widespread

use

in

countries

endemic

with

drug-resistant

tuberculosis.Despite this,these assays have rarely been evaluated in China,where the burden of drug-resistant tuberculosis is among the highest globally.Methods:Mycobacterium tuberculosis clinical isolates were obtained between January 2008 and December 2008.Isolates were tested for drug resistance against rifampicin (RFP) and isoniazid (INH) using the GenoType MTBDRplus assay and drug resistance against ethambutol (EMB),ofloxacin (OFX),and kanamycin (KM) using the Genotype

MTBDRsl

assay.These

results

were

compared

with

conventional drug-susceptibility testing (DST).Results:Readable results were

obtained

from

235

strains

by

GenoType

MTBDRplus

assay.Compared to DST,the sensitivity of GenoType MTBDRplus assay to detect RFP,INH,and multidrug resistance was 97.7％,69.9％,and 69.8％,respectively,whereas the specificity for detecting RFP,INH,and

multidrug resistance was 66.7％,69.2％,and 76.8％,respectively.The sensitivity and specificity of the GenoType MTBDRslassay were 90.9％ and 95.2％ for OFX,77.8％ and 99.5％ for KM,63.7％ and 86.4％ for EMB,respectively.Mutations in codon S531L of the rpoB gene and codon S315T1 of KatG gene were dominated in multidrug-resistant tuberculosis

(MDR-TB)

strains.Conclusions:In

combination

with

DST,application of the GenoType MTBDRplus and MTBDRsl assays may be a useful supplementary tool to allow a rapid and safe diagnosis of multidrug resistance and extensively drug-resistant tuberculosis. 【总页数】8页(1521-1528) 【关键词】

【作者】Qiao Liu;Guo-Li Li;Cheng Chen;Jian-Ming Wang;Leonardo Martinez;Wei Lu;Li-Mei Zhu

【作者单位】Department of Chronic Communicable Disease,Center for Disease Control and Prevention of Jiangsu Province,Nanjing,Jiangsu 210009,China;Department of Chronic Communicable Disease,Center for Disease Control and Prevention of Jiangsu Province,Nanjing,Jiangsu 210009,China;Department of Chronic Communicable Disease,Center for Disease Control and Prevention of Jiangsu Province,Nanjing,Jiangsu 210009,China;Department of Epidemiology and Biostatistics,School of Public

Health,Nanjing

Medical

University,Nanjing,Jiangsu

211166,China;Department of Epidemiology and Biostatistics,University

of Georgia School of Public Health,Athens 21401,Georgia,USA;Center for Global Health,University of Georgia School of Public Health,Athens 21401,Georgia,USA;Department

of

Chronic

Communicable

Disease,Center for Disease Control and Prevention of Jiangsu Province,Nanjing,Jiangsu

210009,China;Department

of

Chronic

Communicable Disease,Center for Disease Control and Prevention of Jiangsu Province,Nanjing,Jiangsu 210009,China 【正文语种】英文 【中图分类】 【文献来源】

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