Effect of environmental factors on chemoresistance of HepG2 cells by regulating hypoxia-in

Effect of environmental factors on chemoresistance of HepG2 cells by regulating

hypoxia-inducible factor-1α

ZHU Hong;LUO Shun-feng;WANG Ji;LI Xin;WANG Hui;PU Wang-yang;ZHANG Hong;ZHUANG Zhi-xiang

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2012(125)006

【摘要】Background Accumulating evidence demonstrates that the microenvironment of the host has an important effect on the chemoresistance of tumors.We also found that the formation of intrinsic multidrug resistance is related to environmental factors that are common with tumor growth of hepatocellular carcinoma.The aim of this study was to explore the molecular mechanisms by which multidrug resistance

of

hepatocellular

carcinoma

is

induced

by

the

microenvironment.in particular,the regulation of nuclear transcription factor (hypoxia-inducible factor-1α,HIF-1α)activation in the process of multidrug resistance formation was investigated.Methods HepG2 cells were

exposed

to

different

microenvironmental

conditions

respectively,such as hypoxia,stimulation of glucose deprivation and transfection of plasmid PcDNA3/HBx.In the HepG2 cells,the expression of the related MDR proteins,HIF-1α protein expression and localization,activity of extracellular signal-regulated kinase /mitogen-