卡莫司汀致皮质发育障碍的模型研究
龙莉莉;肖波;宋延民;王康;李国良
【期刊名称】《中华神经医学杂志》 【年(卷),期】2008(007)005
【摘要】Objective To establish the animal model of diffuse cortical dysplasia in Sprague-Dawley rats. Methods Pregnant rats were given intraperitoneal injections of BCNU on embryonic day 17 (E17). Cresyl-violet staining was applied to observe the histological changes in the cortex, hippocampus and neurons of the resulted pups at P60. EEG recordings were detected, and daily activities were observed. Hot water bath was used to induce seizures, the latency to seizure onset and duration of SE (epileptic status) were compared. Learning and memory abilities were estimated with Y maze at different time points. Results The mean wet brain weights in the BCNU-exposed pups were lower than those of controls on P0 (P＜0.01). Cresyl-violet staining revealed disruption of cortical lamination and heterotopic cell clusters within the hippocampus. Daily activities were poor in BCNU-exposed pups. No obvious epilepsia discharges were detected. After being induced seizures, adult rats with cortical dysplasia had shorter latency to seizures(P＜0.01). The frequency of attempting learning and memory of rats in model group was increased than that in normal group (P＜0.05). Conclusions Intraperitoneal injections of BCNU on embryonic day 17 (E
17) can establish the animal model of diffuse cortical dysplasia, and this model has increased seizure susceptibilities and cognitive functional impairments.%目的 建立SD大鼠广泛型皮质发育障碍的动物模型.方法 在SD大鼠孕17 d腹腔注入BCNU(1,3-二氯乙烯一亚硝基脲,卡莫司汀)制作皮质发育障碍模型;病理检查P60仔鼠皮层和海马结构及神经元变化;行为学观察和脑电图检测;热水浴诱导痫性发作,观察两组大鼠的致痫潜伏期和癫痫持续时间;Y迷宫法测试不同时间点仔鼠学习记忆能力.结果 PO仔鼠脑组织湿重实验组比对照组显著减轻(P<0.01);Nissl染色显示皮质层次紊乱、海马区域异位细胞异常聚集,可出现结节状灰质团块;模型鼠日常活动能力较差,脑电图未见明显痫性放电;模型组热水浴诱导惊厥发作的潜伏期明显缩短(P<0.01);模型组达到学会标准所需电击次数较对照组增加(P<0.05).结论 孕17 d腹腔注射BCNU可建立广泛型皮质发育障碍的动物模型,其致痫敏感性增加,且伴有认知功能障碍. 【总页数】4页(457-460)
【关键词】发育障碍;癫痫;致痫敏感性;认知功能障碍 【作者】龙莉莉;肖波;宋延民;王康;李国良
【作者单位】410008,长沙,中南大学湘雅医院神经内科;410008,长沙,中南大学湘雅医院神经内科;570206,海南省海口市人民医院神经内科;410008,长沙,中南大学湘雅医院神经内科;410008,长沙,中南大学湘雅医院神经内科 【正文语种】中文 【中图分类】R742.1 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-
journal-neuromedicine_thesis/0201237861049.html 【相关文献】
1.皮质发育障碍致癫痫的病理生理机制 [J], 马勋泰; 晏勇 2.皮质发育障碍致癫痫的病理生理机制 [J], 马勋泰; 晏勇
3.局灶性皮质发育障碍致痫的蛋白质组学研究 [J], 黄艳军; 郑帼; 卢孝鹏; 陆海英; 莫绪明
4.卡莫司汀致间质性肺炎一例 [J], 王志伟; 宋伟安
5.甲基氧化偶氮甲醇诱导皮质发育障碍动物模型的研究 [J], 陈兵; 杨小林; 刘立红; 陈欣; 杨辉; 刘仕勇
以上内容为文献基本信息，获取文献全文请下载
卡莫司汀致皮质发育障碍的模型研究
