多次静脉移植人脐血单个核细胞对家兔急性心肌梗死炎症
反应的影响
袁春菊;余国龙;李文斌;杨天伦
【期刊名称】《中华器官移植杂志》 【年(卷),期】2011(032)006
【摘要】Objective To investigate the effects of multi-intravenous transplantation of human cord blood mononuclear cells(HUMNCs)on inflammatory responses and interleukin-6(IL-6),IL-10 after myocardial infarction in rabbits.Methods Totally 45 Chinese rabbits were randomly divided into three groups:multi-transplantation group,single cell transplantation
group,control
group.The
rabbits
in
control
group(n=15),which had undergone ligation of the left anterior coronary artery(LAD),were intravenously injected with normal saline 7,9,11,13 days after the operation.The rabbits in single transplantation group(n=15)were intravenously injected with 3×106 HUMNCs labeled with bromodexyuridine(BrdU)at 7th day after the operation,and normal saline at 9th,11th,13th day post-operation.The rabbits in multiple transplantation group(n=15)were intravenously injected with 3×106 HUMNCs labeled with BrdU at 7th,9th,11th,and 13th day after the operation.In addition,10 Chinese rabbits served as the sham operation group,receiving the operation without ligation of the LAD.Cardiac functions were examined by echocardiography,and pathohistological
多次静脉移植人脐血单个核细胞对家兔急性心肌梗死炎症反应的影响
