Local anesthetic lidocaine induces apoptosis in human corneal stromal cells in vitro

Local anesthetic lidocaine induces apoptosis in

human corneal stromal cells in vitro

Xin Zhou; Yi-Han Li; Hao-Ze Yu; Rui-Xin Wang; Ting-Jun Fan

【期刊名称】《《国际眼科杂志：英文版》》 【年(卷),期】2013(000)006

【摘要】AIM:To demonstrate the apoptosis-inducing effect of iidocalne on human corneal stromal(HCS)cells fn vitm,and provide experimental basis

for

safety

anesthetic

usage

In

clinic

of

ophthalmology.METHODS:In vitro cultured HCS cells were treated with lidocaine at different doses and times,and their morphology was monitored successively with inverted phase contrast microscopy.The membrane permeability of them was detected by acridine orange/ethidium fragmentation

of

bromide(AO/EB)double them

was

examined

staining.The by

agarose

DNA gel

electrophoresis,and their ultrastructure was observed by transmission electron microscopy(TEM),respectively.RESULTS:Exposure to lidocaine at doses from0.3125g/L to 20g/L induced morphological changes of HCS cells such as cytoplasmic vacuolation,cellular shrinkage,and turning round,and elevated membrane permeability of these cells in AO/EB staining.The change of morphology and membrane permeability was doseand time-dependent,while lidocaine at dose below0.15625g/L could not induce these changes.Furthermore,lidocaine induced DNA