不同抗原的酶联免疫斑点检测技术对结核病的辅助诊断价
值比较
刘菲;张宗德;高孟秋;马丽萍;王庆枫;吴晓光;贾红彦;古淑香;马玙
【期刊名称】《中华结核和呼吸杂志》 【年(卷),期】2009(032)011
【摘要】Objective To compare different Mycobacterium tuberculosis antigens in enzyme-linked immunospot assay(ELISPOT)for the auxiliary diagnosis of active tuberculosis.Methods The peripheral blood mononuclear cells(PBMC)from patients with tuberculosis and controls were co-cultured with the following antigens:purified protein derivative(PPD),early secretory antigenic target-6(ESAT-6)and early secretory
antigenic
target-6/culture
filtrate
protein-10
fusion
protein(ESAT-6/CFP-10).Spot forming cells (SFC)were enumerated by ELISPOT. Results PPD-ELISPOT,E/C-ELISPOT and ESAT-6-ELISPOT showed significantly higher SFC counts in active tuberculosis[255(93-526),148(40-354)and 28 (10-116)respectively]as compared to the controls[10(5-41),10(0-20)and
5(0-15)
respectively],μvalues
were
1479.5.1390.5 and 2510.5 respectively,all P＜0.01.Compared with the sensitivity of ESAT-6.ELISPOT(62.1%).that of E/C-ELISPOT and PPD-ELISPOT was higher(90.3%and 84.8%),χ~2=17.496 and 28.541,all P＜0.01.Compared with the specificity of PPD-ELISPOT(68.9%),that of E/C-ELISPOT
and
ESAT-6-ELISPOT
was
also
higher(84.4%and
不同抗原的酶联免疫斑点检测技术对结核病的辅助诊断价值比较
