RP-HPLC法测定人血浆和唾液中奥卡西平活性代谢物的浓
度
冯健全;许俊羽;吴晔;周颖;崔一民
【期刊名称】《中国临床药理学杂志》 【年(卷),期】2013(029)004
【摘要】目的 建立反相高效液相色谱法测定人血浆和唾液中奥卡西平活性代谢物利卡西平(MHD)浓度的方法学.方法 色谱柱为ZORBAX Eclipse Plus C18,柱温为40℃,流速为1 mL·min-1,流动相为甲醇-水=45∶55,检测波长为254nm,内标为对氯苯乙酰胺.结果 血浆及唾液中内源性杂质对样品测定无干扰,血浆及唾液中MHD在2～ 100 μg·mL-1内线性关系良好(血浆中γ=0.999 8,唾液中γ=0.999 8),最低检测浓度为2μg·mL-.血浆中MHD日内、日间RSD均＜5％,提取回收率为72％～89％.唾液中MHD日内、日间RSD均＜5.6％,提取回收率为75％ ～91％.结论 本方法简便、灵敏、准确,适用于人体内奥卡西平活性代谢物MHD的浓度监测.%Objective To establish a HPLC method for determining the concentration of monohydroxycarbamazepine ( MHD) in human plasma and saliva. Methods The analysis was conducted using a ZORBAX Eclipse Plus C18 column and the column temperature was 40 ℃. The mobile phase consisted of methanol and water (45:55). The flow rate was 1 mL ? min-1 and the detection was performed at 254 nm. And the internal standard substance was p - Chloroacetanilide. Results Endogenous impurities does not interfere with the determination of the samples in plasma and saliva. The standard curve is linear in the range
RP-HPLC法测定人血浆和唾液中奥卡西平活性代谢物的浓度
