牙龈卟啉单胞菌脂多糖影响单核细胞表达Th17细胞分化
相关因子的研究
张莉平;高雳;赵雅静;张弛;王兴羽;赵川江
【期刊名称】《中华老年口腔医学杂志》 【年(卷),期】2015(000)001
【摘要】Objective:To investigate the effects of lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg) on the expression of Th17 cell differentiation related cytokines including IL-1β,IL-6,IL-23 and TGF-βin CD14+ monocytes. Methods:The LPS was extracted from standard strain ATCC33277 and high virulent strain W83 by LPS Extraction Kit. The CD14+ monocytes were separated from human fresh peripheral blood by Anti-Human CD14 Magnetic Particles–DM, and then treated with LPS from Pg as well as Escherichia coli (E.coli) in 1μg/ml for 12、24、36 hours. The expression of IL-1β,IL-6,IL-23 and TGF-βmRNA in CD14+ monocytes was detected by Real Time- Polymerase Chain Reaction (RT-PCR). And the protein levels of these cytokines in cell culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA).Results:Pg-LPS and Ecoli-LPS could up-regulate the expression of IL-1β,IL-6,IL-23 and TGF-βboth in mRNA and protein levels in a time-effect manner with peak values at 12h. And there were differences in the expression of the four cytokines among different LPS treated groups.Conclusion:Pg-LPS influenced the expression of Th17 cell
牙龈卟啉单胞菌脂多糖影响单核细胞表达Th17细胞分化相关因子的研究
