Transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci MED and it

Transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci MED and its effects

on host preference of vector whitefly

WEI Ke-ke, LI Jie, DING Tian-bo, LIU Tong-xian, CHU Dong

【摘 要】Abstract The epidemiology of Tomato chlorosis virus (ToCV) in China is closely associated with its vector whitefly, Bemisia tabaci(Gennadius) MED. However, the transmission characteristics of ToCV by B. tabaci MED remain poorly understood. In this study, we analyzed: 1) the horizontal and vertical transmission of ToCV by B. tabaci MED whiteflies； 2) the acquisition of ToCV by male and female B. tabaci MED whiteflies after different feeding durations； 3) the transmission efficacy of viruliferous male and female B. tabaci MED whiteflies after different inoculation access periods (IAPs)； 4) the retention of ToCV by viruliferous male and female B. tabaci MED whiteflies after a 48 h acquisition access period (AAP)； and 5) the effects of ToCV on host choice of healthy or ToCV-infected tomato plant of viruliferous and non-viruliferous B. tabaci MED at different time points. Our results showed that: 1) viruliferous males could not transfer ToCV to non-viruliferous females,and vice versa, viruliferous females could not pass on ToCV to non-viruliferous males. ToCV could not be detected in the F1 generation adults； 2) ToCV could be detected within 4.0% of females or males after a 20 min AAP； 3) ToCV could be