重度间歇低氧大鼠学习记忆功能与氧化应激的关系
王红阳;韩晓庆;赵雅宁;张盼盼;杨林;雷军旗;陈宝元
【期刊名称】《中华结核和呼吸杂志》 【年(卷),期】2012(035)001
【摘要】Objective To observe the relations between learning memory function and oxidative stress in rats exposed to severe intermittent hypoxia (IH).Methods Adult male Wistar rats (n =48)were randomly assigned to 2 groups: untreated control group (UC) and 5％ intermittent hypoxia group (5％ IH).At 2,4,6 and 8 weeks,the learning and memory ability of the rats in each group were assessed with the Morris water maze (MWM) test.The ultrastructural changes in the hippocampus of the rats were observed with transmission electron microscopy (TEM).The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by colorimetric method.Results The water maze test showed that the rats in the 5％ IH group had prolonged latency in escaping,reduced target quadrant time in crossing and reduced frequency of crossing the platform,as compared to the UC group (P ＜ 0.05).With longer time of hypoxia,the damage became more significant.It was observed that the structure of neuron cell was complete and organelles were abundant in UC groups.With the extention of exposure time,the number of hippocampus neurons and synaptic were decreased in 5％ IH groups,nucleus shrank,synaptic frame
重度间歇低氧大鼠学习记忆功能与氧化应激的关系
