4个多拷贝RMY-STR基因座的异常分型
张文琼;肖超;余谨;贾雲舒;黄代新
【期刊名称】《中国法医学杂志》 【年(卷),期】2016(031)005
【摘要】目的：观察湖北汉族人群中4个多拷贝RM Y-STR基因座的异常分型。方法使用文献报道及自行设计的荧光标记引物对252个无关男性个体样本进行扩增，并用AB 3130遗传分析仪对扩增产物进行检测分析。结果在所有样本中共发现25例异常多等位基因分型，其中基因座DYF387S1出现5例，DYF399S1出现15例， DYF403S1出现1例，DYF404S1出现4例。25例异常分型存在于20个样本中，发生率为7.94%，其中4个样本在两个以上基因座上均出现异常分型。结论多拷贝RM Y-STR基因座异常分型发生率较高，在法医学的实际应用中应予以注意。%Objective To observe anomalous genotypes of 4 multi-copy RM Y-STRs in Han population in Hubei province. Methods 252 unrelated male samples were ampliifed using reported and newly designed primers, then detected and analyzed by AB 3130 genetic analyzer. Results A total of 25 anomalous multi-band patterns were observed in 20 samples corresponding to an incident rate of 7.94%. 5 anomalous genotypes were observed in DYF387S1 locus, 15 in DYF399S1, 1 in DYF403S1 and 4 in DYF404S1. Four samples showed extra alleles in more than one locus. Conclusion Anomalous genotype has high incident rates in RM Y-STR markers and requires extensive attention in forensic practice.
4个多拷贝RMY-STR基因座的异常分型
