ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHE

ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL

expression

Xuehuan Dai;Zhenhua Liu;Meng Qiao;Juan Li;Shuo Li;Fengning Xiang

【期刊名称】《植物学报（英文版）》 【年(卷),期】2017(059)010

【摘要】Auxin and cytokinin direct cell proliferation and differentiation during the in vitro culture of plant cells,but the molecular basis of these processes,especially de novo shoot regeneration,has not been fully elucidated.Here,we

describe

the

regulatory

control

of

shoot

regeneration in Arabidopsis thaliana (L.) Heynh,based on the interaction of ARABIDOPSIS RESPONSE REGULATOR12 (ARR12) and WUSCHEL (WUS).The major site of ARR12 expression coincided with the location where the shoot apical meristem (SAM) initiated.The arr12 mutants showed

severely

impaired

shoot

regeneration

and

reduced

responsiveness to cytokinin;consistent with this,the overexpression of ARR12

enhanced

shoot

regeneration.Certain

shoot

meristem

specification genes,notably WUSCHEL (WUS) and CLAVATA3,were significantly

downregulated

(ChIP)

in and

the

arr12

explants.Chromatin activation

assays

immunoprecipitation transient

demonstrated that ARR12 binds to the promoter of WUS.These observations indicate that during shoot regeneration,in vitro,ARR12