Han
Arterioscler Thromb Vasc Biol 34(7): 1522-1530.
Otway, R., et al. (2007). \of atrial fibrillation?\
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Takagi, T., et al. (2007). \295-300.
Tan, J. T., et al. (2009). \variation in KCNQ1 associates with fasting glucose and beta-cell function: a study of 3,734 subjects comprising three ethnicities living in Singapore.\
Thomas, G. N., et al. (1999). \hypertension.\
Tsai, F. J., et al. (2010). \PLoS Genet 6(2): e1000847.
Ullrich, S., et al. (2005). \Unoki, H., et al. (2008). \in KCNQ1 are associated with susceptibility to type 2 diabetes in East Asian and European populations.\
Xu, Y., et al. (2013). \
Yasuda, K., et al. (2008). \in KCNQ1 are associated with susceptibility to type 2 diabetes mellitus.\Nat Genet 40(9): 1092-1097.
Zeggini, E., et al. (2007). \of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes.\Science 316(5829): 1336-1341.
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Zhang, W., et al. (2015). \rs2237892 of KCNQ1 Is Potentially Associated with Hypertension and Macrovascular Complications in Type 2 Diabetes Mellitus in A Chinese Han Population.\Zhang, X., et al. (2012). \of single nucleotide polymorphisms in TCF2 with type 2 diabetes susceptibility in a Han Chinese population.\
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Table. 1 Clinical characteristics of hypertension with T2DM in subjects
Parameters
CC
Age(years) Gender(male/female)
BMI(kg/m2) SBP(mmHg) DBP(mmHg) FPG(mM) TC(mM) TG(mM) LDL-C(mM) HDL-C(mM) HbA1c
57±11.7 2/5 26.44±3.1 140.71±16.3 82.29±8.92 8.19±2.31 4.35±1.14 2.93±3.06 2.64±0.91 0.99±0.15 8.32±1.76
rs2237895
AC+AA 56±8.44 58/29 27.22±3.11 139.33±15.2 85.57±10.98 8.52±2.96 4.17±1.09 3.99±17.85 2.61±0.98 2.63±14.00 8.04±1.35
P value 0.774 0.094 0.529 0.820 0.446 0.774 0.663 0.876 0.932 0.759 0.648
CC 56.43±9.36 32/19 27.18±3.01 138.54±14.34 85.61±10.85 8.74±2.76 4.19±0.97 1.94±1.54 2.66±0.87 1.03±0.23 8.17±1.16
rs2237892
CT+TT 55.69±7.82 28/16 27.09±3.26 140.57±16.42 84.89±10.92 8.18±3.07 4.16±1.24 2.14±1.81 2.55±1.09 1.01±0.19 7.94±1.62
P value 0.452 0.466 0.785 0.123 0.722 0.710 0.102 0.359 0.226 0.719 0.236
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Table. 2 Genotype distribution and Allele frequency of rs2237895 and rs2237892 in KCNQ1 gene in T2DM complicated with HBP patients
SNPs Group Genotype distribution and frequency P value
OR (95%CI)
1.9551(0.753-5.077)
Allele frequency P value 0R (95%CI)
AA
case
rs2237895
48(0.511)
AC 39(0.415)
CC 7(0.074)
0.359
A 135(0.718)
C 53(0.282)
0.215
1.311(0.854-2.013)
control 47(0.456) 42(0.408) 14(0.136) 136(0.660) 70(0.340)
rs2237892
case
CC 56(0.549)
CT 38(0.373)
TT 8(0.078)
0.167
1.699(0.977-2.952)
C 150(0.735)
T 54(0.265)
0.098
1.43(0.956-2.185)
control 43(0.417) 50(0.485) 10(0.097) 136(0.660) 70(0.340)
Table. 3 Haplotype analysis of rs2237895 and rs2237892 in KCNQ1 associated with
hypertension with T2DM by case control comparison
Haplotype homozygous wild type heterozygous wild type Sum up
homozygous+heterozygous
Wild type
105(0.558) 83 (0.441)
67 (0.325) 139(0.674)
Case 26(0.277) 15(0.159) 53(0.564) 15(0.159)
control 12(0.117) 48(0.680) 43(0.417) 48(0.466)
P value 6×10-6
4×10-5
1.6×10-6
2.62 (1.742-3.954)
OR 6.93 (2.828-16.997)
3.94 (1.948-7.988)
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Table. 4 Association between genotypes of rs2237895, rs2237892 and clinician parameters
rs2237895
rs 2237892
rs2237895
rs 2237892
Parameters
P value
P value
Parameters
P value
P value
AC+AA CC
CC CT+TT AC+AA CC CC CT+TT Gender
Age (years)
Male 15 2 11 6 ≧60 15 2 11 6 Female
16 2
1.00 10 8
0.302
< 60 13 3
0.656
8 8
0.211
BMI(kg/m2)
BMI(kg/m2)
≧25 18 2 12 8 ≧25 15 4 11 8 < 25 6 2 0.555 6 2 0.669 < 25 3 1 1.00 3 1 1.00 SBP (mmHg)
SBP (mmHg)
≧140 14 2 10 6 ≧140 11 4 11 4 < 140 15 2 1.00 10 7 0.420 < 140 14 1 0.330 7 8 0.264 DBP (mmHg) DBP (mmHg)
≧ 90 5 0 3 2 ≧ 90 5 2 6 1 < 90 24 4 1.00 17 11 1.00 < 90 20 3 0.565 12 11 0.193 FPG (mM)
FPG (mM)
≧ 7.0 19 3 12 10 ≧ 7.0 16 4 12 8 < 7.0 10 1 1.00 8 3 0.456 < 7.0 9 1 0.640 6 4 1.00 TC (mM)
TC (mM)
≧ 5.72 0 2 3 1 ≧ 5.72 2 2 2 2 < 5.72 29 2 0.010 19 14 0.633 < 5.72 23 3 0.119 10 16 1.00 TG (mM)
TG (mM) ≧ 1.7. 8 3 8 3 ≧ 1.7. 9 2 7 4 < 1.7 21 1 0.097 12 10 0.456 < 1.7 16 3 1.00 0 8 0.03 LDL-C (mM)
LDL-C (mM)
≧ 3.3 5 2 5 2 ≧ 3.3 7 2 5 4 < 3.3 24 2 0.190 15 11 0.431 < 3.3 18 3 0.622 13 8 1.00 HDL-C(mM)
HDL-C(mM)
≧1.0/1.3 7 0 5 2 ≧1.3 3 0 2 1 < 1.0/1.3 21 4 1.00 15 10 0.683 < 1.3 21 5 1.00 16 10 0.684 HbA1c (%)
HbA1c (%)
≧6.5 22 2 14 10 ≧6.5 21 4 16 9 < 6.5
0 1
0.068
1 0
1.00
< 6.5
0 0
1.00
0 0
1.00
a. Association of genotypes with parameters in over 60 group
b. Association of genotypes with parameters in female group
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rs2237895
rs 2237892
Parameters
Pvalue
P value
AC+AA CC CC CT+TT Age (years)
≧60 16 2 10 8 < 60 35 0
0.119
18 17
0.393
BMI(kg/m2)
≧25 37 1 20 18 < 25 11 1 0.426 8 4 0.512 SBP (mmHg)
≧140 28 0 11 17 < 140 23 2 0.336 17 8 0.021 DBP (mmHg)
≧ 90 23 0 11 12 < 90 28 2 0.623 17 13 0.269 FPG (mM)
≧ 7.0 36 1 20 17 < 7.0 15 1 0.517 8 8 0.397 TC (mM)
≧ 5.72 5 0 2 3 < 5.72 46 2 0.417 26 22 0.658 TG (mM) ≧ 1.7. 19 2 12 9 < 1.7 31 0 0.295 15 16 0.276 LDL-C (mM)
≧ 3.3 12 0 7 5 < 3.3 39 2 1.00 21 20 0.242 HDL-C(mM)
≧1.0 20 0 10 10 < 1.0 31 2 1.00 15 18 0.379 HbA1c (%)
≧6.5 36 1 11 7 < 6.5
6 1
0.296
1 3
0.127
c. Association of genotypes with parameters in male group
Han
Figure. 1 Genotyping results for rs2237892 and rs2237895 in KCNQ1 gene
a. Genotyping result for rs2237892
CC
TT CT
b. Genotyping result for rs2237895
AC
700 bp
AA
CC
400 bp
200 bp
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