福建省布鲁氏菌分离株的分子生物学鉴定
邓艳琴;王加熊;林代华;陈亮;王灵岚
【期刊名称】《中国人兽共患病学报》 【年(卷),期】2009(025)007
【摘要】AMOS-PCR and MLVA were carried out to identify the Brucella strains isolated in Fujian province, which were classified as B. melitensis biovar 2 or 3 by conventional microbiological tests. All these 3 isolates were identified to be B. melitensis by AMOS-PCR. The genetic patterns obtained by MLVA were queried in the Brucella 2007 database and clustered with B. melitensis strains. It is evident that these two molecular assays may be used as the assistant tools in the identification of Brucella strains.%目的 利用分子生物学方法鉴定福建省布鲁氏菌分离株.方法 对布鲁氏菌分离株进行AMOS-PCR和MLVA分型.结果 3株福建省分离株经AMOS-PCR鉴定为羊种,与传统生物学分型一致;MLVA分型将其分为3个基因型,聚类分析鉴定为羊种.结论 AMOS-PCR、MLVA分型可以作为我省布鲁氏菌分离株鉴定的辅助手段. 【总页数】4页(636-639)
【关键词】布鲁氏菌;鉴定;AMOS-PCR;MLVA 【作者】邓艳琴;王加熊;林代华;陈亮;王灵岚
【作者单位】福建省疾病预防控制中心,福州,350001;福建省疾病预防控制中心,福州,350001;福建省疾病预防控制中心,福州,350001;福建省疾病预防控制中心,福州,350001;福建省疾病预防控制中心,福州,350001
福建省布鲁氏菌分离株的分子生物学鉴定
