Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology

Three-dimensional cultures of human endometrial

cells on Matrigel mimic in vivo morphology

ZHU Hai-yan;WANG Jun-xia;TONG Xiao-mei;XU Wei-hai;JIANG Ling-ying;JING Xiao-ying;YANG Ling-yun;ZHOU Feng;ZHANG Song-ying

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2012(125)005

【摘要】Background The regulation of endometrial physiology and morphogenesis by the paracrine effectors has been well established using in vivo studies.A more complete understanding of the endometrial function has been delayed due,in part,to a lack of appropriate culture models.In this study,we aimed to simulate the in vivo three-dimensional (3-D) growth pattern of endometrial cells using a 3-D in vitro culture system.Methods Isolated endometrial epithelial cells,stromal cells and RL95-2 cells were seeded into culture chambers coated with the extracellular matrix Matrigel and observed using light microscopy.Fluorescence staining and immunohistochemistry were used to assess the morphology.Results Depending on the culture conditions,epithelial cells and RL95-2 cells formed multicellular structures

on

Matrigel; or

grew

stromal together

cells to

remained form

3-D

individually lattice-like

distinguishable

structures.Conclusions Matrigel provided a good microenvironment for culturing endometrial cells.The cells cultured in the Matrigel-coated

chambers closely resembled those seen in vivo. 【总页数】6页(863-868) 【关键词】

【作者】ZHU Hai-yan;WANG Jun-xia;TONG Xiao-mei;XU Wei-hai;JIANG Ling-ying;JING Xiao-ying;YANG Ling-yun;ZHOU Feng;ZHANG Song-ying 【作者单位】Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, College of Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China;Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, College of Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China;Assisted Reproduction Unit, Department of Obstetrics and

Gynecology,

Sir

Run

Run

Shaw

Hospital,

College

of

Medicine,Zhejiang University, Hangzhou, Zhejiang 310016,

China;Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, College of Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China;Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, College of Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China;Assisted Reproduction Unit, Department of Obstetrics and

Gynecology,

Sir

Run

Run

Shaw

Hospital,

College

of

Medicine,Zhejiang University, Hangzhou, Zhejiang 310016,

China;Assisted Reproduction Unit, Department of Obstetrics and

Gynecology, Sir Run Run Shaw Hospital, College of Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China;Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, College of Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China;Assisted Reproduction Unit, Department of Obstetrics and

Gynecology,

Sir

Run

Run

Shaw

Hospital,

College

of

Medicine,Zhejiang University, Hangzhou, Zhejiang 310016, China 【正文语种】中文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-medical-journal_thesis/0201254505876.html 【相关文献】

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