MWNTs与GNPs-CHIT修饰的过氧化氢生物传感器
马霄;谢国明;周觅;邹超世;王艳;甄生航
【期刊名称】《传感器与微系统》 【年(卷),期】2009(028)010
【摘要】Multi-walled nanotubes(MWNTs) is self-assembled onto the coated glassy carbon elecrtode(GCE) to form a negative-charge surface. Cationic dye-thionine (Thi) is linked by electrostatic adsorption as an electron transfer mediator. Next the mixture of gold nanoparticles ( GNPs) and chitosan ( CHIT) is assembled on the electrode by covalent bond. The positively charged horseradish peroxidase is successfully immobilized on the GNPs surface. The electrocataltyic behavior of the modified electrode to H_2O_2 is investigated by cyclic voltammetry and chronoamperometry. The experiments show that the modified electrode has excellent electrocatalytic activity for the detection of H2_O_2. The linear range of this biosensor is 8.2 ×10~(-6)-1.1 ×10~(-3) mol/L with a detection limit of 5.8 ×10~(-7) mol/L. The response time achieved 95 % steady state is about 15 s. The biosensor is applied for deteetion of H_2 O_2 , and the results are satisfied.%在玻碳电极(GCE)上自组装一层多壁碳纳米管(MWNTs),构建负电荷的界面,然后,静电吸附一层阳离子电子媒介体硫堇(Thi),再由共价键作用自组装一层纳米金(GNPs),壳聚糖(CHIT)混合溶液的复合薄膜,通过静电吸附辣根过氧化物酶(HRP)制得过氧化氢(H_2O_2)生物传感器.采用循环伏安法和计时电流法考察了该生物传感器的电化学性质,并研究了该修饰
MWNTs与GNPs-CHIT修饰的过氧化氢生物传感器
