Differential Expression of a Glycogen Phosphorylase Gene in Volvariella volvacea Mycelium

Differential Expression of a Glycogen Phosphorylase Gene in Volvariella volvacea Mycelium Exposed to Low Temperature Stress

ZHAO Yan;WANG Hong;LI Zhengpeng;LU Lianjing;CHEN Mingjie

【期刊名称】《食用菌学报》 【年(卷),期】2014(000)004

【摘要】Relative expression of a glycogen phosphorylase gene (pyg)in mycelia of cold-sensitive (V23)and cold-tolerant (VH3)strains of Volvariella volvacea during exposure to low temperature (0 ℃)over time courses was quantified by real-time PCR using theα-tubulin gene as internal control.Pyg expression levels in strain V23 decreased after 4 h exposure and,although recovering after 6 h,still remained lower than untreated controls.Gene expression in strain VH3 decreased sharply after low temperature exposure for 2 h, reaching a minimum value after 8 h when the relative expression level was only 0.28 times that of untreated controls.Overall,although pyg expression decreased in both V23 and VH3 over prolonged exposure,the fall was less pronounced in strain V23 compared with VH3. 【总页数】5页(5-9) 【关键词】

【作者】ZHAO Yan;WANG Hong;LI Zhengpeng;LU Lianjing;CHEN Mingjie