RP-HPLC法测定晕痛定胶囊中阿魏酸的含量
刘文苹;吴小菲;李军;孟祥松
【期刊名称】《世界最新医学信息文摘（电子版）》 【年(卷),期】2013(000)008
【摘要】 目的建立用高效液相色谱法测定晕痛定胶囊中阿魏酸的含量。方法 Hypersil ODS 2.5um(4.6mm×250mm)色谱柱，以乙腈-0.8%冰醋酸（17：83）为流动相，流速为1.0ml/min，检测波长为320nm，进样量为10μl。结果阿魏酸在5.60μg～33.60μg范围内线性良好，回归方程Y=26452X+21918，r=0.9991(n=6)。平均回收率(n=6)为99.25%（RSD=0.47%）。结论本方法简便快捷，稳定性高，重现性好，为制定晕痛定胶囊的质量控制标准提供参考依据。%Objective to establish a set of ferulic acid content determination of
dizzy
pain
capsule
by
hplc.Methods:Hypersil
ODS
2.5μm(4.6mm×250mm) chromatographic column,acetonitrile-0.8%
glacial acetic acid (17:83)as mobile Phase ,the lfow rate was 1.0ml/min,the detection wavelength was 320nm,10μl sample.Result ferulic acid was 5.60μg/ml ~ 33.60μg/ml good linear range,regression equation was Y=26452X+21918,r=0.9991(n=6) .The average recovery rate (n=6) was 99.25%(RSD=0.47%).Conclusion the method is simple ,fast,high stability ,good reproducibility ,to provide reference basis for the quality control standard of yuntongding capsule. 【总页数】2页(234-235)
【关键词】HPLC;晕痛定胶囊;阿魏酸;含量测定
RP-HPLC法测定晕痛定胶囊中阿魏酸的含量
