Imaging of apoptotic HeLa cells by using scanning near-field optical microscopy

Imaging of apoptotic HeLa cells by using scanning

near-field optical microscopy

佚名

【期刊名称】《自然科学进展（英文版）》 【年(卷),期】2002(012)008

【摘要】By using scanning near-field optical microscopy (SNOM), HeLa cells in apoptosis process are imaged with a higher optical resolution beyond the diffraction limit. Since SNOM provides both topographic and transmitted light intensity information of a cell, it can correlate the structural characteristics and optical properties with the spatial position of the apoptotic cells. Wavelength imaging by using near-field spectroscopy shows that there is a great difference in light propagation and absorption in the cell. This unique technique can be applied to the super high resolution imaging of different components in the cell. The observations by near-field optical imaging and near-field spectroscopy indicate an inhomogeneous aggregation of the inner structure in the apoptotic HeLa cells and the change of transmission intensity of light with the apoptosis status. 【总页数】6页(577-582) 【关键词】scanning

near-field

optical

microscopy;HeLa

cell;apoptosis;spectrum imaging;tapping mode 【作者】佚名