A comparative study on the transplantation of different concentrations of human umbilical

A comparative study on the transplantation of different concentrations of human umbilical

mesenchymal cells into diabetic rats

Jia-Hui;Kong;Dan;Zheng;Song;Chen;Hong-Tao;Duan;Yue-Xin;Wang;Meng;Dong;Jian;Song;

【期刊名称】《国际眼科杂志：英文版》 【年(卷),期】2015(000)002

【摘要】AIM: To observe the effects of intravitreal injections of different concentrations of human umbilical mesenchymal stem cells on retinopathy in rats with diabetes mellitus.METHODS: Healthy and adult male Sprague-Dawley(SD) rats were randomly assigned to a normal control group(group A), a diabetic retinopathy(DR) blank control group(group B), a high-concentration transplantation group(group C), a low-concentration transplantation group(group D) and a placebo transplantation group(group E). The expression of nerve growth factor(NGF)protein

in

the

retinal

layers

was

detected

by

immunohistochemical staining at 2, 4, 6 and 8wk.RESULTS: The expression of NGF was positive in group A and most positive in the retinal ganglion cell layer. In groups B and E, the expression of NGF was positive 2wk after transplantation and showed an increase in all layers. However, the level of expression had decreased in all layers at 4wk and was significantly reduced at 8wk. In groups C and D, the expression of