EZH2基因在他莫昔芬耐药乳腺癌MCF-7细胞系的表达及
其对细胞增殖的影响
骆广涛;施航;王本忠
【期刊名称】《中华乳腺病杂志（电子版）》 【年(卷),期】2015(000)001
【摘要】Objective To investigate the expression of EZH2 gene in tamoxifen-resistant breast cancer MCF-7 cells ( MCF-7/Tam+) and tamoxifen-sensitive breast cancer MCF-7 cells( MCF-7/Tam-) , and explore the effects of EZH2 on the proliferation of the two breast cancer cell lines. Methods Firstly, the expressions
of EZH2 in MCF-7/Tam+ and MCF-7/Tam- cells were detected by Western blot. After MCF-7/Tam- cells being treated by 10 nmol/l estradiol and 10 nmol/l 4-hydroxy tamoxifen(4-OHT) for 24 h, the expression of EZH2 protein was detected by Western blot. siRNA with targeted inhibition of EZH2 (
siRNA-EZH2
)
was
transfected
into
MCF-7/Tam+
using
LipofectamineTM 2000. The real-time PCR and Western blot were used to detect the mRNA and protein expression of EZH2 respectively in order to confirm the validity of siRNA. Finally, siRNA-EZH2 was transfected into MCF-7/Tam-and MCF-7/Tam+cells. Cell count assay was used to measure the cell number and MTT to detect the cell proliferation ( optical density at 570 nm wavelength ) . Measurement data were expressed as x±s, and t test was used to compare the means.
EZH2基因在他莫昔芬耐药乳腺癌MCF-7细胞系的表达及其对细胞增殖的影响
