Effect of Bcl-2 and caspase-3 on calcium distribution in apoptosis of HL-60 cells

Effect of Bcl-2 and caspase-3 on calcium distribution in apoptosis of HL-60 cells

佚名

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2000(000)003

【摘要】Apoptosis manifests in two major execution programs downstream of the death signal: the caspase pathway and organelle dysfunction. An important antiapoptosis factor, Bcl-2 protein, contributes in caspase pathway of apoptosis. Calcium, an important intracellular signal element in cells, is also observed to have changes during apoptosis, which maybe affected by Bcl2 protein. We have previously reported that in Harringtonine (HT) induced apoptosis of HL-60 cells, there's a change of intracellular calcium distribution, moving from cytoplast especially Golgi's apparatus to nucleus and accumulating there with the highest concentration. We report here that caspase-3 becomes activated in HT-induced apoptosis of HL-60 cells, which can be inhibited by overexpression of Bcl-2 protein. No sign of apoptosis or intracellular calcium movement from Golgi's apparatus to nucleus in HL-60 cells overexpressing Bcl-2 or treated with Ac-DEVD-CHO, a specific inhibitor of caspase-3. The results indicate that activated caspase-3 can promote the movement of intracellular calcium from Golgi's apparatus to nucleus, and the process is inhibited by Ac-DEVD-CHO (inhibitor of

caspas-3), and that Bcl-2 can inhibit the movement and accumulation of intracellular calcium in nucleus through its inhibition on caspase3. Calcium relocalization in apoptosis seems to be irreversible, which is different from the intracellular calcium changes caused by growth factor. 【总页数】8页(213-220)

【关键词】Apoptosis, calcium, caspase-3, bcl-2, laser scan ning confocal microscopy (LSCM). 【作者】佚名

【作者单位】Department of Biology, Beijing Normal University, Beijing 100875, China;Department of Biology, Beijing Normal University, Beijing 100875, China;Department of Biology, Beijing Normal University, Beijing 100875, China 【正文语种】中文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_cell-research-english_thesis/0201255255136.html 【相关文献】

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